Journal: bioRxiv

Article Title: Stereotypic Neutralizing V H Clonotypes Against SARS-CoV-2 RBD in COVID-19 Patients and the Healthy Population

doi: 10.1101/2020.06.26.174557

Figure Lengend Snippet: Recombinant SARS-CoV-2 RBD-coated microtiter plates were incubated with varying concentrations of scFv-hCκ fusion proteins. HRP-conjugated anti-human Ig kappa light chain antibody was used as the probe, and TMB was used as the substrate. All experiments were performed in duplicate, and the data are presented as the mean ± SD.

Article Snippet: A phage display of the human scFv libraries was subjected to four rounds of biopanning against the recombinant SARS-CoV-2 S and RBD proteins (Sino Biological Inc., Beijing, China), fused to mFc or hCκ, as described previously .

Techniques: Recombinant, Incubation

Journal: bioRxiv

Article Title: Stereotypic Neutralizing V H Clonotypes Against SARS-CoV-2 RBD in COVID-19 Patients and the Healthy Population

doi: 10.1101/2020.06.26.174557

Figure Lengend Snippet: The recombinant scFv-hFc fusion proteins (200 nM or 600 nM) were mixed and incubated with recombinant SARS-CoV-2 S glycoprotein (200 nM) fused with a HIS tag at the C-terminus. After incubation with Vero E6 (ACE2 + ) cells, the relative amount of bound, recombinant SARS-CoV-2 S glycoprotein was measured using a FITC-conjugated anti-HIS antibody. For each sample, 10,000 cells were monitored.

Article Snippet: A phage display of the human scFv libraries was subjected to four rounds of biopanning against the recombinant SARS-CoV-2 S and RBD proteins (Sino Biological Inc., Beijing, China), fused to mFc or hCκ, as described previously .

Techniques: Recombinant, Incubation

Journal: bioRxiv

Article Title: Stereotypic Neutralizing V H Clonotypes Against SARS-CoV-2 RBD in COVID-19 Patients and the Healthy Population

doi: 10.1101/2020.06.26.174557

Figure Lengend Snippet: The recombinant scFv-hCκ fusion proteins were mixed with 2,500 TCID 50 of SARS-CoV-2 (BetaCoV/Korea/SNU01/2020, accession number MT039890 ), and the mixture was added to the Vero cells. After 0, 24, 48, and 72 h of infection, the culture supernatant was collected to measure the viral titers.

Article Snippet: A phage display of the human scFv libraries was subjected to four rounds of biopanning against the recombinant SARS-CoV-2 S and RBD proteins (Sino Biological Inc., Beijing, China), fused to mFc or hCκ, as described previously .

Techniques: Recombinant, Infection

Journal: bioRxiv

Article Title: Stereotypic Neutralizing V H Clonotypes Against SARS-CoV-2 RBD in COVID-19 Patients and the Healthy Population

doi: 10.1101/2020.06.26.174557

Figure Lengend Snippet: Recombinant wild-type or mutant (V341I, F342L, N354D, V367F, R408I, A435S, G476S, and V483A) SARS-CoV-2 RBD protein-coated microtiter plates were incubated with varying concentrations of scFv-hFc fusion proteins. HRP-conjugated anti-human IgG antibody was used as the probe, and ABTS was used as the substrate. All experiments were performed in triplicate, and the data are presented as the mean ± SD.

Article Snippet: A phage display of the human scFv libraries was subjected to four rounds of biopanning against the recombinant SARS-CoV-2 S and RBD proteins (Sino Biological Inc., Beijing, China), fused to mFc or hCκ, as described previously .

Techniques: Recombinant, Mutagenesis, Incubation

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: List of ELISA tests, cell proliferation, and protein extraction kits used. For ELISA tests, dilution of the supernatants or cell lysate samples used and detection limits are also reported.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Enzyme-linked Immunosorbent Assay, Protein Extraction

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: C-Kit (CD117) mRNA (a, b, c) and protein (d, e, f) expression after ESW treatment in primary bronchial fibroblasts of COPD patients (a, d), primary bronchial fibroblasts of control smokers (b, e), and bronchial epithelial cells (c, f). In bronchial epithelium (16HBE) c-Kit increased at mRNA (c) and protein (f) levels. In primary bronchial fibroblasts of COPD patients, c-Kit increased at protein level (d). T -test was used for comparative purposes, and p values are reported in the graphs.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Expressing

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: Photomicrographs showing thyroid transcription factor-1 (TTF-1) expression (panels a, b), c-Kit (CD117) (c, d), and proliferating cell nuclear antigen (PCNA) (e, f) in the peripheral lung tissue of a representative patient with chronic obstructive pulmonary disease (COPD). Arrows indicate positively stained cells mainly located in the alveolar septa. Bars = 50 microns.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Expressing, Staining

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: Photomicrographs showing alveolar type II epithelial cells (TTF-1+ cells, red color) coexpressing c-Kit (CD117) (brown color) (a, b) and PCNA (brown color) (c, d) in the peripheral lung tissue of a representative patient with COPD. Positive double-stained cells can be recognized in the alveolar septa, even though their presence was only rarely observed. Arrows indicate positively stained cells located in the alveolar septa. Bars = 50 microns.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Staining

Journal: Biology Open

Article Title: Apolipoprotein CIII regulates lipoprotein-associated phospholipase A 2 expression via the MAPK and NFκB pathways

doi: 10.1242/bio.201410900

Figure Lengend Snippet: (A–C) THP-1 cells were incubated with 30 µg of apo CIII for the indicated durations. MCP-1, TNF-α and IL-6 were detected in the medium using an ELISA kit ( n = 3). (D–F) THP-1 cells were pretreated with 1-linoleoyl glycerol (75 µM) for 1 h and incubated with or without apo CIII (30 µg) for the indicated durations. The secretion of MCP-1, TNF-α and IL-6 was assayed using an ELISA kit ( n = 3). Error bars represent mean±s.e.m. *** P <0.0001.

Article Snippet: The levels of proinflammatory cytokines which stimulated by apo CIII and released in the culture media were evaluated by Human IL-6 ELISA Kit, Human TNFα ELISA Kit and Human MCP-1 ELISA Kit (Boster Inc., China), following the manufacturer's instructions.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay

Journal: Virus Research

Article Title: Identification and characterization of linear B cell epitopes on the nucleocapsid protein of porcine epidemic diarrhea virus using monoclonal antibodies

doi: 10.1016/j.virusres.2020.197912

Figure Lengend Snippet: Reactivity of McAb and mice sera against PEDV YZ and SH strain by iELISA. Four synthesized polypeptides were coated in the ELISA plate, respectively. McAb and mice sera were added into the wells and then and HRP-labeled Goat Anti-Mouse IgG (H + L) (Beyotime, China) was added into each well. After developing colour with TMB substrate (Beyotime, China) and stoping with 2 M H 2 SO 4, absorbance was read on Multiscan Spectrum (Epoch, Biotek) at 450 nm. PEDV SH: mice sera against PEDV SH strain; PEDV YZ: mice sera against PEDV YZ strain; 1C9: McAb 1C9.

Article Snippet: To identify the subtype of the McAb, a commercial ELISA kit (Proteintech) was used.

Techniques: Synthesized, Enzyme-linked Immunosorbent Assay, Labeling

Journal: American Journal of Cancer Research

Article Title: Antineoplastic efficacy profiles of avapritinib and nintedanib in KIT D816V + systemic mastocytosis: a preclinical study

doi:

Figure Lengend Snippet: Specification of monoclonal antibodies (mAb)

Article Snippet: SCF-R/KIT , CD117 , 104D2 , mouse , IgG1 , PE , BD Biosciences.

Techniques: